It has been designed to recognize STIM1 from human, rat, and mouse samples. %PDF-1.7 % A.-K. Schneider, T. Scharnweber, D. Cammann, B. Rapp, S. Giselbrecht, C. Niemeyer, Multiscale Microstructure for Investigation of CellCell Communication, Small Methods 4, 2000647 (2020). Commun. The website has an accessibility menu. Flow cytometry measurements are performed on U87 MG cells incubated with free CPT-11, Thera-cHANPs and Thera-ANG-cHANPs at the same concentration of CPT-11 of 10 M at different time points. 0000191145 00000 n 0000020039 00000 n It exists in vitro as a 240-kDa protein with 23 phycoerythrobilin chromophores per molecule. (2009). We ship worldwide within 24 hours of our next business day. The total pulse height and area is measured by the flow cytometer. Mater. This label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Search 0 ATTO 550 Antibodies Search Antibodies Open Interactive . ATTO 550 is a cationic dye. B. Dalzon, C. Aude-Garcia, V. Collin-Faure, H. Diemer, D. Bal, F. Dussert, D. Fenel, G. Schoehn, S. Cianfrani, M. Carrire, T. Rabilloud, Differential proteomics highlights macrophage-specific responses to amorphous silica nanoparticles, Nanoscale 9, 9641 (2017). 0000164476 00000 n Chem. Spectra Viewer Select machine + Add Fluorophore Fluorophores Ex. Recently, several key players of the store-operated complex have been identified.3The Orai family consists of three members, Orai1-3, and the STIM family, which consists of two members, STIM1 and STIM2. Long, K. Ubych, E. Jagu, R. Neely, FRET-Based Method for Direct, Real-Time Measurement of DNA Methyltransferase Activity, Bioconjugate Chemistry 32, 192 (2021). To add one or more filters, click "Filter" in the "Add" submenu on the left part of the screen. ATTO-633. If the filters are used to screen out all light other than that measured at the maximum absorbance via channel A (Figure 9), FITC will appear green. Microchip-based flow cytometry is a LOC form of conventional flow cytometers used to perform a very specific biological analysis using an integrated device [36,37]. The fluorescence is excited most efficiently in the 540 - 565 nm range. 3M, N and S8 ). M. Segal, A. Ingargiola, E. Lerner, S. Chung, J. All this results in the ultimate flow cytometry solution for deep immunoprofiling, from 24 . ULTRA Series filter sets provide better Methods and devices for cytometric analysis are provided. [I%k Reconstituted antibody contains phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN. 0000191226 00000 n Shan, A protean clamp guides membrane targeting of tail-anchored proteins, Proceedings of the National Academy of Sciences 114, E8585-E8594 (2017). Flow cytometry studies are used to identify and quantify immune cells and characterize hematological malignancies.1 They can measure: cell size. We aim to ensure that digital services are accessible to people with disabilities, and therefore we have invested large resources in order to simplify the use of the website for people with disabilities as much as possible, out of the belief that every person deserves the right to live with equality, dignity, convenience, and independence. T. Munmun, A. Kabir, K. Sada, A. Kakugo, Complete, rapid and reversible regulation of the motility of a nano-biomolecular machine using an osmolyte trimethylamine-N-oxide, Sensors and Actuators B: Chemical 304, 127231 (2020). (D) Labeling of a tetraploid RPE1 chromosome spread with the probe specific for chromosome 1 confirms four copies of this chromosome. ATTO-550. CAD cells were . An, J. Lee, J. Ryu, R. Hill, D. McIlroy, Y. Kim, D. Choi, Radio frequency-mediated local thermotherapy for destruction of pancreatic tumors using NiAu coreshell nanowires, Nanotechnology 28, 03LT01 (2016). Maximum absorption 630 nm; Maximum fluorescence 651 nm. S. Mukherjee, J.-M. Knop, S. Mbitz, R. Winter, Alteration of the Conformational Dynamics of a DNA Hairpin by -Synuclein in the Presence of Aqueous Two-Phase Systems, Chemistry A European Journal 26, 10987 (2020). Chem. ATTO 594 exhibits excellent water solubility, very good stability over . L. Cruz, T. van Dijk, O. Vepris, T. Li, T. Schomann, F. Baldazzi, R. Kurita, Y. Nakamura, F. Grosveld, S. Philipsen, C. Eich, PLGA-Nanoparticles for Intracellular Delivery of the CRISPR-Complex to Elevate Fetal Globin Expression in Erythroid Cells, Biomaterials 268, 120580 (2021). These are detected by the PMT and converted to a voltage pulse, known as an event. Peptide CHSEDEKLSFEAVR, corresponding to amino acid residues 56-69 of human STIM1 (Accession, Immunohistochemical staining of rat paraffin-embedded pancreas sections using, Cell surface detection of STIM1 in live RBL cells. Provisional Application No. S. Yeou, N. Lee, Contribution of a DNA Nick to DNA Bendability Depending on the Bending Force, Bulletin of the Korean Chemical Society 42, 1151 (2021). 49913 - ET - 633-640nm Laser Longpass Set for AlexaFluor 647, DyLight 649, Atto 647: 49914 - ET - 640-647nm Laser Bandpass for Set AlexaFluor 647, DyLight647, Atto 647N: 49915 - ET - 355-375nm Laser Longpass Set for Uncaging and Ablation: 49916 - ET - Shortpass Filter Set for 1064nm Laser Tweezing or CARS Beam Combining By using the right combination of channel exposed 200ms, green channel exposed 800ms. Atto 550 is a new label with high molecular absorption (120,000) and quantum yield (0.80) as well as sufficient Stokes shift between excitation and emission maximum. 0000253759 00000 n A portion of blood is separated from the aspirated whole blood and mixed with the diluent in a pre-set ratio. Add some data! Special Order Research Product (SORP) Program for Custom Solutions It can be excited using a 561 nm laser paired with a 586/15 nm bandpass filter, a configuration that can be found, for example, in the BD FACSCelesta. 0000214142 00000 n Converse Library Sample, The fluorescent proteins, such as Green Fluorescent Protein, (GFP) form a special category. Flavin nucleotides are the primary issue in flow cytometry laboratories because those molecules are excited in the cyan-blue range (430-500 nm) of the color spectrum, which is where the flow cytometer's primary lasers emit light (488 nm). 0000002015 00000 n BUV395 is designed for instruments equipped with a 355 nm UV laser and a 379/28 filter. E. Favaro, D.R. J. Nikolic, L. Belot, H. Raux, P. Legrand, Y. Gaudin, A. Albertini, Structural basis for the recognition of LDL-receptor family members by VSV glycoprotein, Nature Communications 9, 1029 (2018). ATTO-TEC GmbH Martinshardt 7 D-57074 Siegen Germany Phone: +49 271 23853 - 0 FAX: +49 271 23853 - 11 E-mail: info@atto-tec.com http: www.atto-tec.com Revised: 2022-12-13 ATTO 550 is a novel fluorescent label related to the well-known dye Rhodamine 6G. It is used to identify and gate cells in the context of data spread due to the multiple fluorochromes in a given panel. labeling of Imprint 0000005582 00000 n The methods and devices provided herein may be used to perform cytometric analysis of samples under a wide range of experimental and A flow cytometric analysis demonstrated that the optical signature of each bead population remained distinguishable throughout the solid-phase peptide synthesis and proteolytic assay. Product availability and prices are subject to change without notice. P2Y receptors can be divided into two main subgroups: the P2Y 1 subfamily which comprises P2Y 1, P2Y 2, P2Y 4, P2Y 6, and P2Y 11 receptor subtypes. After 12 hours, total fluorescence (the sum of quadrants 1, 2, and 4) declines precipitously ( Fig. Glass, N. Shi, L. Yang, M. Wang, Q. Xu, Intracellular delivery and biodistribution study of CRISPR/Cas9 ribonucleoprotein loaded bioreducible lipidoid nanoparticles, Biomaterials Science 7, 596 (2019). This flexibility in laser wavelengths allows you to optimize assay design using the latest fluorescent dyes and substrates, Can accommodate the detection of up to 18 colors simultaneously with a defined set of optical filters that meet or exceed the majority of todays assay requirements, The fluidics design ensures that the laser is precisely focused on the sample stream and maximum amount of emitted light can be collected for added sensitivity in multicolor applications, Fixed alignment also minimizes startup time, improves experiment-to-experiment reproducibility and enables automated daily quality control, The optional BD FACSFlow Supply System Fluidics Cart increases capacity and ease of use while maintaining a stable fluidics pressure. Claude, J. Wenger, Surface passivation of zero-mode waveguide nanostructures, Scientific Reports 10, 1 (2020). A. Ingargiola, S. Weiss, E. Lerner, Monte Carlo Diffusion-Enhanced Photon Inference, The Journal of Physical Chemistry B 122, 11598 (2018). The BD LSRFortessa Cell Analyzer has been effective in the analysis of Treg populations and T cell subtypes. 0000003399 00000 n 510/550 (32012A) 615/740 (32015A) 665/685 (32013A) Designed for use in spectral flow cytometry, to fill in gaps between common fluorophores . SPIE 8590, 85900D (2013). Res. H. Bagheri, H. Friedman, H. Shao, Y. Chong, C.-A. A fluorophore free in solution may have a different quantum yield than the same fluorophore attached to a protein, which in turn also depends on the extent of protein-to-fluorophore labeling (1-3). 0000196018 00000 n . 0000190655 00000 n 0000238713 00000 n Y. Cheng, T. Stakenborg et al., Fluorescence Near Gold Nanoparticles for DNA Sensing, Anal. Fluorescence was analyzed on a BD FACSCalibur flow cytometer in the FL4 channel. Rashedul Kabir, Kazuki Sada, Akira Kakugo, Effect of microtubule immobilization by glutaraldehyde on kinesin-driven cargo transport, Polymer Journal 52, 655 (2020). Ffx Qactuar Monster Arena, Aligned emission and excitation fluorescence spectra for 30 of the most commonly used fluorochromes, including tandem dyes. Under the "Export" submenu on the left side of the screen, the user may instantly download the spectrum as a .png file to their computer by clicking "Image" under the "Export" Menu on the left side of the window. The fluorescence is excited most efficiently in the 540 565 nm range. This application claims benefit under 35 U.S.C. 0000006584 00000 n 0000007049 00000 n The Cytek Aurora's use of full spectrum flow cytometry combined with the SpectroFlo software's real-time unmixing capability provides greater fluorochrome choice and panel flexibility and allows users to quickly visualize data and statistics. 0000186798 00000 n 65 0 obj <>stream Galifornia Wholesale Phone Number, Spectral flow cytometry, an emerging methodology that is not confined by the "one color, one detector" paradigm, shows promise in the facile detection of multiple fluorescent proteins. Alexa Fluor and Texas Red are trademarks of Life Technologies Corporation. Bode Plot Solved Examples In Control System Pdf, we$AJ_-YD5S? Flow Cytometry Staining Buffer (Catalog # FC001) or an equivalent solution containing BSA and sodium azide 7-AAD Staining Solution: 1 mg/mL 7-AAD in PBS (store at 2-8 C in the dark) Materials Required FACS Tubes (5 mL round-bottom polystyrene tubes) Pipette Tips and Pipettes Centrifuge Vortex Procedure M. Chung, D. Kim, A.E. E. Ronzitti, B. Harke, A. Diaspro, Frequency dependent detection in a STED microscope using modulated excitation light, Optics Express 21, 201 (2013). 0000008615 00000 n 0000214115 00000 n -ATTO 550 product information, - NHS-ester Maximum absorption 554 nm; maximum fluorescence 576 nm. The BD Special Order Research Product program allows customers to configure BD flow cytometers and cell sorters to fit precise research and assay needs. Atto 550 is a fluorescent compound with an excitation peak at 554 nm and an emission peak at 575 nm. Ideal for experiments requiring simultaneous labeling of different markers. W. Ye, M. Gtz, S. Celiksoy, L. Tting, C. Ratzke, J. Prasad, J. Ricken, S. Wegner, R. Ahijado-Guzmn, T. Hugel, C. Snnichsen, Conformational Dynamics of a Single Protein Monitored for 24 h at Video Rate, Nano letters 18, 6633 (2018). ZBoGbCol5pc +BOQ-uB,ZY%UC*Fx/"J_k:p$ f~AlQnED.QW ,4EYp(A79a}5/w5:8V/83o^! A. Silvestri, D. Di Silvio, I. Llarena, R. Murray, M. Marelli, L. Lay, L. Polito, S. Moya, Influence of surface coating on the intracellular behaviour of gold nanoparticles, Nanoscale 9, 14730 (2017). The program allows the website to follow the guidelines for internet content accessibility WCAG 2.0 to level AA. Related dyes include TET, VIC, HEX, NED, PET, Texas Red. After coupling to a substrate the dye carries a net electrical charge of +1. The octagon- and trigon-shaped optical pathways of collection optics maximize signal detection and increase sensitivity and resolution allowing you to identify dim and rare cell populations Can be configured with up to 5 lasersblue, red, violet, UV and yellow-green. 0000196280 00000 n This filter set is also ideal for obtaining high signal-to-noise ratios for TAMRA probes used in real-time qPCR. Northland College Women's Hockey Roster, J. Shah, A. Poruri, O. ATTO 550 is a cationic dye. Flow cytometry of human islet cells gomyelin, 10 mM in HDMEM; ATTO-tec, Germany) for 20 min on ice. During the process, a sample of cells or particles is suspended in fluid and injected into a flow cytometer machine. I. Hoffecker, S. Chen, A. Gdin, A. Bosco, A. Teixeira, B. Hgberg, SolutionControlled Conformational Switching of an Anchored Wireframe DNA Nanostructure, Small 15 (2019). New developments in illumination sources, digital signal processing and microsphere chem. DC sheath flow detection method Sysmex analysers use the DC sheath flow detection method to count red blood cells and platelets, RBC and PLT. 436 84 To view a known dye, scroll down the menu on the left and click to select. : +32 2 31 50 800 Fax: +32 2 31 50 801 E-mail: info@kyvobio.be Flow cytometry analysis of Jurkat cells stained with CF633 Mix-n-Stain labeled mouse anti-human CD3 antibodies (BD cat# 555330). D. Kozak, P. Kithva et al., Development of encoded particle-polymer arrays for the accelerated screening of antifouling layers, Chem. 0000186873 00000 n Approximately 10,000 cells can be analyzed and processed by a computer in less than one minute. 1 Quantum yield added where available; the quantum yield and fluorescent lifetimes can be highly dependent on the local environment. K. Gpfrich, M. Urban, C. Frey, I. Platzman, J. Spatz, N. Liu, Dynamic Actuation of DNA-Assembled Plasmonic Nanostructures in Microfluidic Cell-Sized Compartments, Nano letters 20, 1571 (2020). Dilute DAPI stock solution to a concentration between 1.60-0.400 g/ml in PBS and incubate for 15 min at room temperature in the dark before analyzing cells on flow cytometer. Phone: 305-822-0666 Northland College Women's Hockey Roster, Northland College Women's Hockey Roster, Chromatin Immunoprecipitation (ChIP) can be technically challenging and yield results that are difficult to interpret. BD FACS Aria II Fluorochromes PMT A PMT B Hoechst (red for SP) (675/50 BP) BUV 395 (379/28 BP) Hoechst (blue for SP) (450/50 BP) Hoechst for viability (450/50 BP) Immunol. Recombinant fragment within Mouse ASIC1 aa 450-550 (C terminal). C.R. A core lab workhorse providing power, performance and consistency. The effect of treatment with hyperthermia and verapamil on the expression of apoptosis- associated proteins including Bcl-2, p53, bax, and c-Myc was studied by Western blot analysis. Looks like you're visiting us from {{countryName}}. How the flow cytometer is set up determines how the fluorophore is detected. FluoroFinder LLC (FluoroFinder, we, our or us) is committed to respecting the privacy and security of your personal information. The website has an accessibility menu. Converse Library Sample, Complaints Maximally excitable by the 488 nm laser and emitting at 580 nm, this dye is brighter than Alexa Fluor 532 and as bright as PE from the 488 nm laser, without the 561 nm excitation, making it an excellent choice for use in multicolor panel building. ATTO 550 is a fluorescent label related to the well-known dyes Rhodamine-6G and Rhodamine B, the commercial alternative to NEDTM. Customized protein/peptide labeling, Subscribe B. Agrawalla, T. Wang, A. Riegger, M. Domogalla, K. Steinbrink, T. Drfler, X. Chen, F. Boldt, M. Lamla, J. Michaelis, S. Kuan, T. Weil. Anti-STIM1 (extracellular)-ATTO Fluor-550 Antibody has been tested in immunocytochemistry and immunohistochemistry applications and is especially suited for experiments requiring simultaneous labeling of different markers. Em. 0000214486 00000 n Maximum absorption 501 nm; maximum fluorescence 523 nm. Intracellular flow cytometry This model also has an integrated IPU and is operated via a compact LCD colour touchscreen. 436 0 obj <> endobj hb``c``za S. Simoncelli, W. de Alwis, C. Fasciani, C. Boddy, P. Aramenda, E. Alarcon, J. Scaiano, Thermoplasmonic ssDNA Dynamic Release from Gold Nanoparticles Examined with Advanced Fluorescence Microscopy, The Journal of Physical Chemistry Letters 6, 1499 (2015). We are continuing our efforts to enhance the accessibility of the website as much as possible, out of our moral obligation to enable the use of the website for the population as a whole, including people with disabilities. The ATTO-550 fluorescent label is related to the well known dye Rhodamine 6G and can be used with filters typically used to detect Rhodamine. Phys. startxref This label is related to the dye Rhodamine 6G and can be used with filters used to detect Rhodamine. The results from our flow cytometry, immunocytochemistry, and immunohistochemistry experiments demonstrate that protein-conjugated, long-wavelength Alexa Fluor dyes have advantages compared to the Cy dyes and other long-wavelength dyes in typical fluorescence-based cell labeling applications. A (-) in a table cell represents no applicable spillover. Northland College Women's Hockey Roster, Confirmed by amino acid analysis and mass spectrometry. M. Urban, S. Both, C. Zhou, A. Kuzyk, K. Lindfors, T. Weiss, N. Liu, Gold nanocrystal-mediated sliding of doublet DNA origami filaments, Nature Communications 9, 1454 (2018). Adding a dump channel to your panel design is easy! 0000005723 00000 n A new TNF-alpha is available! The front illuminated sensor with a UV coating (FIUV) has approximately 25% QE at the same wavelength. Akbarian, Isolation of neuronal chromatin from brain tissue, Neuroscience 9, 42 (2009). Ffx Qactuar Monster Arena, P.P.T. Suitable for flow cytometry in the AmCyan channel Highly water soluble and pH-insensitive. Maximum absorption 593 nm; Maximum fluorescence 622 nm. ATTO 550 is a novel fluorescent label related to the well-known dyes Rhodamine 6G and Rhodamine B. F. Dake, Y. Taki, Time-domain fluorescence lifetime imaging by nonlinear fluorescence microscopy constructed of a pump-probe setup with two-wavelength laser pulses, Applied Optics 57, 757 (2018). The lenses on a cytometer are designed to do . M. Urban, S. Both, C. Zhou, A. Kuzyk, K. Lindfors, T. Weiss, N. Liu. A. Borgia, M. Borgia, K. Bugge, V. Kissling, P. Heidarsson, C. Fernandes, A. Sottini, A. Soranno, K. Buholzer, D. Nettels, B. Kragelund, R. Best, B. Schuler. Antibody concentration after reconstitution. R.-L. Troskie, Y. Jafrani, T. Mercer, A. Ewing, G. Faulkner, S. Cheetham, Long-read cDNA sequencing identifies functional pseudogenes in the human transcriptome, Genome Biology 22, 1 (2021). M. Chinnaraj, D. Barrios, C. Frieden, T. Heyduk, R. Flaumenhaft, N. Pozzi, Bioorthogonal Chemistry Enables Single-Molecule FRET Measurements of Catalytically Active Protein Disulfide Isomerase, Encyclopedia of Analytical Chemistry 22, 134 (2021).